The bumper delivery marks the most successful breeding season on record for the not-for-profit organisation since it began building an insurance population three years ago.

The Eastern quoll has been extinct on the Australian mainland since the 1960s due to trapping, poisoning and feral pests including cats and foxes.

Tasmanian quoll numbers had also declined more than 50 per cent in the decade to 2009, with an estimated 10,000 left in that state.

"Eastern quolls were once common throughout Australia, but sadly can now only be found in the wild in Tasmania," Aussie Ark president Tim Faulkner said.

"They are an important part of our ecosystem with their appetite for rabbits, mice and rats keeping the populations of these pests under control."

The quoll pouch count follows a record 69 joeys for the Tasmanian devils in their Barrington sanctuary.